PAR/PAK® II ADP, Collagen, & Epinephrine

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PAR/PAK® II: Streamlining Platelet Studies with ADP, Collagen, and Epinephrine Reagents Combo Kit

PAR/PAK® II (ADP, Collagen, & Epinephrine) is a convenience kit containing platelet aggregation reagents routinely used to elicit responses in Platelet Rich Plasma.

Provides ADP, Collagen, and Epinephrine for Basic Aggregation Responses in Platelet Rich Plasma Samples
Simplifies Laboratory Inventory Control
Conveniently Packaged for Platelet Studies

Catalog No.: 101310

Net Contents: ADP 2 x 0.5 mL

Collagen 2 x 0.5 mL

Epinephrine 2 x 0.5 mL

The PAR/PAK® II Combo Kit is a comprehensive solution for platelet aggregation studies, bringing together three essential reagents—ADP, Collagen, and Epinephrine—in a single, streamlined package. This kit is designed to facilitate both routine and advanced platelet studies in Platelet Rich Plasma (PRP), offering researchers and clinicians a versatile tool for evaluating platelet function.

Each reagent is provided in precise 2 x 0.5 mL vials, ensuring accuracy and consistency in testing. ADP, Collagen, and Epinephrine are key in inducing characteristic aggregation responses in PRP, making them instrumental in identifying both normal and abnormal platelet behavior. These reagents play a critical role in diagnosing and studying platelet function disorders, helping to uncover insights into inherited and acquired platelet abnormalities.

By consolidating these reagents, the PAR/PAK® II Combo Kit enhances lab efficiency and inventory management, ensuring that the necessary components are readily available for platelet studies. With meticulous packaging and standardized volumes, this kit provides a reliable, convenient solution for labs focused on achieving precision and adaptability in platelet function testing.

Explore Product Data Sheet

Discover Expected Results Chart

Carefully explore the documentation on this page, incorporating the PAR/PAK® II Combo Kit Product Data Sheet. Additionally, discover the PAR/PAK® II Combo Kit Instruction for Use (IFU) and the PAR/PAK® II Combo Kit Safety Data Sheet (SDS), both provided in six languages. Thoroughly inspect the PAR/PAK® II Combo Kit EU and UK Declaration of Conformity to guarantee adherence and a detailed understanding, specifically customized for clients and distributors in Europe and the United Kingdom. To comprehend the product's details and specifications comprehensively, it is necessary to review this information diligently.

Bio/Data Corporation’s Aggregation Reagents: Accessible Safety Data Sheets for Secure Handling

Bio/Data Corporation provides comprehensive Safety Data Sheets (SDS) for each of its carefully formulated aggregation reagents. These essential documents ensure safe handling, storage, and spill management while offering clear instructions on potential hazards. Designed for easy viewing, saving, and printing, the SDS are readily accessible to support a safe work environment and responsible chemical management.

Access Safety Data Sheet

Product Description

PAR/PAK® II is a Platelet Aggregation Combination Kit containing ADP (Adenosine-5’- Diphosphate), Collagen (Soluble Calf Skin, Type 1), and Epinephrine (Adrenaline) Reagents. ADP Reagent is a lyophilized preparation of Adenosine-5’-Diphosphate. It is an essential component in platelet aggregation.

ADP acts as an agonist or activator, binding to platelet receptors and triggering a series of biochemical events that lead to platelet activation and aggregation.

Review Instructions For Use

Collagen Reagent is a lyophilized preparation of Soluble Calf Skin (Type 1). Collagen Reagent induces platelet shape change and activates platelets. The activated platelets then release thrombotic compounds from their granules, which serve to recruit additional platelets to an injury site.

Epinephrine Reagent is a stabilized and lyophilized preparation of L-Adrenaline that activates the GP IIa adreno receptor causing platelet aggregation without shape change. Although it can enhance the response of platelets to other agonists, Epinephrine Reagent is a weak (reversible) agonist. It may or may not elicit a response in healthy people.

PAR/PAK II Combo Kit has been optimized for use with Light Transmission Aggregometers. It may also be used with other turbidometric or impedance analyzers, and flow cytometers.

Intended Purpose

PAR/PAK II Combo Kit is a convenience kit containing a combination of routine platelet aggregation reagents used to elicit aggregation and / or agglutination responses in Platelet Rich Plasma (PRP). The Kit includes ADP, Collagen, and Epinephrine Reagents.

Detection / Measurement

PAR/PAK II Combo Kit Reagents are used, in conjunction with other diluents and control samples, to measure changes of the light transmission in a Platelet Rich Plasma (PRP) test sample.

Product Function

PAR/PAK II Combo Kit provides insight into different aspects of platelet function / quality. This Kit aids in accessing various acquired and inherited platelet disorders or the efficacy of anti-platelet therapies.

Test Principle

When introduced to a stirred, 37°C Platelet Rich Plasma (PRP) test sample, exogenous Reagents such as ADP, Collagen, and Epinephrine stimulate platelets, prompting them to undergo shape change and aggregate. This initial aggregation is called primary aggregation and is reversible. However, normal platelets possess the ability to release endogenous ADP from their granules, leading to a secondary, irreversible wave of aggregation. The Light Transmission Platelet Aggregometer effectively captures these changes by displaying parameters such as lag phase, shape change, and the rate and extent of aggregation over a predetermined testing period.

For Epinephrine, hyper-reactivity may be demonstrated. If so, the Sticky Platelet Procedure should be followed for confirmation. Not all healthy people will respond to Epinephrine Reagent.

READ MORE... Easily Download the PAR/PAK II Combo Kit Instructions for Use in Your Selected Language.

Reagents Provided

ADP Reagent, 2 x 0.5 mL.

Collagen Reagent, 2 x 0.5 mL.

Epinephrine Reagent, 2 x 0.5 mL.

Reagents and Materials Required but Not Provided

Purified Water (Distilled, Deionized, Reagent Grade), pH 5.3 – 7.2 for reconstitution
TRIS Buffered Saline (TBS) or 0.85% physiological saline for dilutions

NOTE: USING BLOOD BANK SALINE WILL CAUSE ERRONEOUS RESULTS.

Materials and Accessories

Platelet Aggregometer
Centrifuge
Electronic Pipette
Pipette Tips
Aggregometer Test Tubes (Siliconized)
Aggregometer Stir Bars (Plastic Coated)
Plastic Sample Tubes and Caps (For Dilutions)

Reagent Storage

ADP, Collagen, and Epinephrine, Reagents do not require temperature protection during shipment.
Upon receipt, store ADP, Collagen, and Epinephrine Reagents at 2 – 8° C in their original packaging.
Reconstituted ADP, Collagen, and Epinephrine Reagents are stable for 30 days when stored in their tightly capped, original containers at 2 – 8° C.

PAR/PAK II Platelet Aggregation Reagent Combo Kit

CONVENIENTLY

PACKAGED TOGETHER

Specific Information Provided

PAR/PAK II Combo Kit Reagents are not intended for the detection of a specific disorder, condition, or risk factor.

ADP Reagent plays a pivotal role in platelet activation and aggregation. When ADP binds to specific receptors on the platelet surface, such as P2Y1 and P2Y12, it initiates intracellular signaling cascades. This activation induces rapid changes in platelet shape and the release of calcium ions through P2Y1 receptors, while P2Y12 activation sustains the response, ensuring stable aggregation. ADP Reagent is utilized to stimulate platelet activation and aggregation precisely by interacting with these ADP receptors. By observing platelet aggregation in response to ADP, clinicians can assess platelet function / quality related to abnormalities in platelet activation and aggregation. This process is crucial for understanding clot formation dynamics and evaluating the efficacy of anti-platelet therapies in preventing thrombotic events. ADP prompts the release of secondary mediators like Thromboxane A2 (TX A2 ), further amplifying platelet activation and aggregation.

Collagen Reagent initiates platelet activation and aggregation. Upon binding to glycoprotein receptors on the platelet surface, particularly glycoprotein VI (GP VI), Collagen sets off intracellular signaling cascades. This triggers rapid changes in platelet shape and the release of calcium ions through GP VI receptors, with sustained activation facilitated by integrin α2β1, ensuring stable aggregation. Utilized to precisely stimulate platelet activation and aggregation, Collagen Reagent interacts with these receptors, providing a means for clinicians to assess platelet function / quality and disorders linked to collagen-induced platelet activation abnormalities. This process is vital for comprehending clot formation dynamics and evaluating the efficacy of anti-platelet therapies inhibiting thrombotic events. Collagen prompts the release of secondary mediators, further amplifying platelet activation and aggregation.

Epinephrine Reagent plays a pivotal role in platelet activation and aggregation. Upon binding to specific receptors on the platelet surface, particularly α2-adrenergic receptors, epinephrine initiates intracellular signaling cascades. This cascade induces rapid changes in platelet shape and triggers the release of calcium ions, crucially mediated through α2-adrenergic receptor activation. The sustained response, essential for stable aggregation, is facilitated by α2-adrenergic receptor activation. Epinephrine Reagent is instrumental in precisely stimulating platelet activation and aggregation by interacting with these adrenergic receptors. Observing platelet aggregation in response to Epinephrine Reagent allows clinicians to assess and evaluate platelet function / quality and disorders associated with abnormalities in platelet activation and aggregation. This process is pivotal for comprehending clot formation dynamics and evaluating the effectiveness of anti-platelet therapies in preventing thrombotic events. Epinephrine prompts the release of secondary mediators, further amplifying platelet activation and aggregation.

Bio/Data Corporation's Commitment to IVDR Compliance
Bio/Data Corporation proudly complies with the stringent EU In Vitro Diagnostic Regulation (IVDR) 2017/746 and other relevant legislation. Through its Declaration of Conformity (DOC), the company ensures that its devices meet essential health, safety, and environmental standards.

Bio/Data Corporation's Commitment to UKCA Compliance
Bio/Data Corporation ensures product excellence by strictly adhering to UKCA regulations. The company's Declaration of Conformity (DOC) affirms its dedication to meeting health, safety, and environmental standards outlined in UKCA directives and any related updates, demonstrating its unwavering commitment to compliance.

View EU Declaration of Conformity

View UK Declaration of Conformity

PAR/PAK® II ADP, Collagen, & Epinephrine

PAR/PAK® II: Streamlining Platelet Studies with ADP, Collagen, and Epinephrine Reagents Combo Kit

PAR/PAK® II (ADP, Collagen, & Epinephrine) is a convenience kit containing platelet aggregation reagents routinely used to elicit responses in Platelet Rich Plasma.

Provides ADP, Collagen, and Epinephrine for Basic Aggregation Responses in Platelet Rich Plasma Samples

Simplifies Laboratory Inventory Control

Conveniently Packaged for Platelet Studies