Catalog No.: 101580

Net Contents: ADP 1 x 0.5 mL

Collagen 1 x 0.5 mL

Ristocetin 1 x 0.5 mL

BETA/PAK® ADP, Collagen, & Ristocetin

< Return To Reagents

BETA/PAK® Combo Kit: ADP, Collagen, Ristocetin for Advanced Platelet Aggregation Studies

BETA/PAK® (ADP, Collagen, and Ristocetin) is a convenience kit containing a combination of routine platelet aggregation reagents used to elicit responses in Platelet Rich Plasma as well as an agglutination response that may be induced by the Ristocetin Reagent.

Provides ADP and Collagen for Basic Aggregation Responses in Platelet Rich Plasma Samples
Includes Ristocetin which Induces Platelet Agglutination in the Presence of vWF
Simplifies Laboratory Inventory Control
Conveniently Packaged for Platelet Studies

The BETA/PAK® Combo Kit combines three essential platelet aggregation reagents—ADP, Collagen, and Ristocetin—in a single, convenient package crafted to meet the needs of both routine and specialized platelet studies. This streamlined kit is designed to simplify laboratory workflows and facilitate in-depth research, offering flexibility for a wide range of platelet function tests.

Each reagent is provided in a precise 0.5 mL volume, ensuring consistency and accuracy across tests. This kit supports critical testing processes such as the Ristocetin Induced Platelet Aggregation Test (RIPA) and the Ristocetin Cofactor Activity Test, which utilizes lyophilized platelets to assess von Willebrand Factor (vWF) function. ADP and Collagen are essential for routine platelet aggregation testing, helping to induce typical aggregation responses in Platelet Rich Plasma (PRP), while Ristocetin is key for studying platelet agglutination, an important factor in vWF analysis.

Explore Product Data Sheet

Discover Expected Results Chart

By consolidating these reagents into a single kit, the BETA/PAK® Combo Kit enhances lab efficiency and simplifies inventory management, providing researchers and clinicians with a reliable, comprehensive solution for adaptable and thorough platelet function testing.

Please explore the provided documentation on this page, including the BETA/PAK Combo Kit Product Data Sheet and Routine Agonist Expected Results Chart. Additionally, consult the BETA/PAK Combo Kit Instruction for Use (IFU) and the BETA/PAK Combo Kit Safety Data Sheet, which are available in six languages. For our European and United Kingdom customers and distributors, thoroughly review the BETA/PAK Combo Kit EU Declaration of Conformity and the BETA/PAK Combo Kit UK Declaration of Conformity to ensure compliance and a nuanced understanding tailored for these specific regions. This information is essential for gaining a comprehensive understanding of the product details and specifications.

Bio/Data Corporation’s Aggregation Reagents: Accessible Safety Data Sheets for Secure Handling

Bio/Data Corporation provides comprehensive Safety Data Sheets (SDS) for each of its carefully formulated aggregation reagents. These essential documents ensure safe handling, storage, and spill management while offering clear instructions on potential hazards. Designed for easy viewing, saving, and printing, the SDS are readily accessible to support a safe work environment and responsible chemical management.

Access Safety Data Sheet

Product Description

BETA/PAK® is a Platelet Aggregation Combination Kit containing ADP (Adenosine-5’- Diphosphate), Collagen (Soluble Calf Skin, Type 1), and Ristocetin (Ristocetin A Sulfate) Reagents.

ADP Reagent is a lyophilized preparation of Adenosine-5’-Diphosphate. It is an essential component in platelet aggregation. ADP acts as an agonist or activator, binding to platelet receptors and triggering a series of biochemical events that lead to platelet activation and aggregation.

Review Instructions For Use

Collagen Reagent is a lyophilized preparation of Soluble Calf Skin (Type 1). Collagen Reagent induces platelet shape change and activates platelets. The activated platelets then release thrombotic compounds from their granules, which serve to recruit additional platelets to an injury site.

Ristocetin Reagent is a lyophilized preparation of Ristocetin A Sulfate, a glycopeptide of unknown chemical structure which has been isolated from Nocardia lurida. Ristocetin contains in excess of 90% Ristocetin A.

BETA/PAK Combo Kit has been optimized for use with Light Transmission Aggregometers. It may also be used with other turbidometric or impedance analyzers, and flow cytometers.

Intended Purpose

BETA/PAK Combo Kit is a convenience kit containing a combination of routine platelet aggregation reagents used to elicit responses in Platelet Rich Plasma (PRP) as well as an agglutination response that may be induced by the Ristocetin Reagent. The Test Kit includes ADP, Collagen, and Ristocetin Reagents.

Detection / Measurement

BETA/PAK Combo Kit Reagents are used, in conjunction with other diluents and control samples, to measure changes of the light transmission in a Platelet Rich Plasma (PRP) test sample.

Product Function

BETA/PAK Combo Kit provides insight into different aspects of platelet function / quality. This Kit aids in accessing various acquired and inherited platelet disorders or the efficacy of anti-platelet therapies.

Test Principle

When introduced to a stirred, 37°C Platelet Rich Plasma (PRP) test sample, exogenous Reagents such as ADP, Arachidonic Acid, Collagen, Epinephrine, and Ristocetin stimulate platelets, prompting them to undergo shape change and aggregate. This initial aggregation is called primary aggregation and is reversible. However, normal platelets possess the ability to release endogenous ADP from their granules, leading to a secondary, irreversible wave of aggregation. The Light Transmission Platelet Aggregometer effectively captures these changes by displaying parameters such as lag phase, shape change, and the rate and extent of aggregation over a predetermined testing period.

READ MORE... Easily Download the BETA/PAK Combo Kit Instructions for Use in Your Selected Language.

Reagents Provided

ADP Reagent, 1 x 0.5 mL.

Collagen Reagent, 1 x 0.5 mL.

Ristocetin Reagent, 1 x 0.5 mL.

Reagents and Materials Required but Not Provided

Purified Water (Distilled, Deionized, Reagent Grade), pH 5.3 – 7.2 for reconstitution
TRIS Buffered Saline (TBS) or 0.85% physiological saline for dilutions

NOTE: USING BLOOD BANK SALINE WILL CAUSE ERRONEOUS RESULTS.

Materials and Accessories

Platelet Aggregometer
Centrifuge
Electronic Pipette
Pipette Tips
Aggregometer Test Tubes (Siliconized)
Aggregometer Stir Bars (Plastic Coated)
Plastic Sample Tubes and Caps (For Dilutions)

Reagent Storage

ADP, Collagen, and Ristocetin Reagents do not require temperature protection during shipment.
Upon receipt, store ADP, Collagen, and Ristocetin Reagents at 2 – 8° C in their original packaging.
Reconstituted ADP and Collagen Reagents are stable for 30 days when stored in their tightly capped, original containers at 2 – 8° C.
Reconstituted Ristocetin Reagent is stable for 7 days, when stored in its’ tightly capped, original container at 2 – 8° C.

Beta/Pak Platelet Aggregation Reagent Combo Kit

CONVENIENTLY

PACKAGED TOGETHER

Specific Information Provided

BETA/PAK Combo Kit Reagents are not intended for the detection of a specific disorder, condition, or risk factor.

ADP Reagent plays a pivotal role in platelet activation and aggregation. When ADP binds to specific receptors on the platelet surface, such as P2Y1 and P2Y12, it initiates intracellular signaling cascades. This activation induces rapid changes in platelet shape and the release of calcium ions through P2Y1 receptors, while P2Y12 activation sustains the response, ensuring stable aggregation. ADP Reagent is utilized to stimulate platelet activation and aggregation precisely by interacting with these ADP receptors. By observing platelet aggregation in response to ADP, clinicians can assess platelet function / quality related to abnormalities in platelet activation and aggregation. This process is crucial for understanding clot formation dynamics and evaluating the efficacy of anti-platelet therapies in preventing thrombotic events. ADP prompts the release of secondary mediators like Thromboxane A2 (TX A2 ), further amplifying platelet activation and aggregation.

Collagen Reagent initiates platelet activation and aggregation. Upon binding glycoprotein receptors on the platelet surface, particularly glycoprotein VI (GP VI), Collagen sets off intracellular signaling cascades. This triggers rapid changes in platelet shape and the release of calcium ions through GP VI receptors, with sustained activation facilitated by integrin α2β1, ensuring stable aggregation. Utilized to precisely stimulate platelet activation and aggregation, Collagen Reagent interacts with these receptors, providing a means for clinicians to assess platelet function / quality and disorders linked to collagen-induced platelet activation abnormalities. This process is vital for comprehending clot formation dynamics and evaluating the efficacy of anti-platelet therapies inhibiting thrombotic events. Collagen prompts the release of secondary mediators, further amplifying platelet activation and aggregation.

Ristocetin Reagent is a distinctive platelet reagent employed in the realm of Ristocetin Induced Platelet Aggregation (RIPA) testing. Ristocetin interacts with von Willebrand Factor (vWF), a critical plasma protein involved in platelet adhesion and aggregation processes. Ristocetin prompts a conformational shift in vWF, exposing binding sites for platelet glycoprotein Ib (GP Ib). Consequently, platelet GP Ib receptors engage with vWF, initiating platelet adhesion. This initial adherence primes platelets for aggregation. In instances lacking von Willebrand Factor (vWF) or related platelet function disorders, Ristocetin Induced Platelet Aggregation proceeds to a limited extent due to platelets’ incapacity to aggregate effectively. Consequently, RIPA testing furnishes invaluable insights into platelet function / quality and vWF activity, thereby aiding in the characterization of von Willebrand Disease (vWD) and associated bleeding disorders. This testing method plays a vital role in evaluating platelet function / quality accurately

Bio/Data Corporation's Commitment to IVDR Compliance
Bio/Data Corporation proudly complies with the stringent EU In Vitro Diagnostic Regulation (IVDR) 2017/746 and other relevant legislation. Through its Declaration of Conformity (DOC), the company ensures that its devices meet essential health, safety, and environmental standards.

Bio/Data Corporation's Commitment to UKCA Compliance
Bio/Data Corporation ensures product excellence by strictly adhering to UKCA regulations. The company's Declaration of Conformity (DOC) affirms its dedication to meeting health, safety, and environmental standards outlined in UKCA directives and any related updates, demonstrating its unwavering commitment to compliance.

View EU Declaration of Conformity

View UK Declaration of Conformity

BETA/PAK® ADP, Collagen, & Ristocetin

BETA/PAK® Combo Kit: ADP, Collagen, Ristocetin for Advanced Platelet Aggregation Studies

BETA/PAK® (ADP, Collagen, and Ristocetin) is a convenience kit containing a combination of routine platelet aggregation reagents used to elicit responses in Platelet Rich Plasma as well as an agglutination response that may be induced by the Ristocetin Reagent.

​

Provides ADP and Collagen for Basic Aggregation Responses in Platelet Rich Plasma Samples

Includes Ristocetin which Induces Platelet Agglutination in the Presence of vWF

Simplifies Laboratory Inventory Control

Conveniently Packaged for Platelet Studies