Product Function
Epinephrine Reagent provides insight into different aspects of platelet function / quality. This Reagent aids in accessing various acquired and inherited platelet disorders or the efficacy of anti-platelet therapies.
Specific Information Provided
Epinephrine Reagent is not intended for the detection of a specific disorder, condition, or risk factor.
Epinephrine Reagent plays a pivotal role in platelet activation and aggregation. Upon binding to specific receptors on the platelet surface, particularly α2-adrenergic receptors, epinephrine initiates intracellular signaling cascades. This cascade induces rapid changes in platelet shape and triggers the release of calcium ions, crucially mediated through α2-adrenergic receptor activation. The sustained response, essential for stable aggregation, is facilitated by α2-adrenergic receptor activation. Epinephrine Reagent is instrumental in precisely stimulating platelet activation and aggregation by interacting with these adrenergic receptors. Observing platelet aggregation in response to Epinephrine Reagent allows clinicians to assess and evaluate platelet function / quality and disorders associated with abnormalities in platelet activation and aggregation. This process is pivotal for comprehending clot formation dynamics and evaluating the effectiveness of anti-platelet therapies in preventing thrombotic events. Epinephrine prompts the release of secondary mediators, further amplifying platelet activation and aggregation.
Automation
Epinephrine Reagent is intended for use in semi-automated and automated Light Transmission Platelet Aggregometers. This Reagent may also be used with other turbidometric or impedance analyzers, and flow cytometers.
Quality / Quantity
There are no primary standards for Epinephrine Reagent. The responses to this reagent is concentration dependent. A known normal donor should be tested with each new lot of Epinephrine Reagent. Standards organizations classify Epinephrine induced platelet aggregation as semi-quantitative or semi-qualitative.
Epinephrine Reagent comes packaged as 3 x 0.5 mL vials. The working concentration of Epinephrine is 100 µM.
Specimen Type
The test specimen is prepared from sodium citrate anti-coagulated whole blood. The test sample is Platelet Rich Plasma (PRP). The test blank is Platelet Poor Plasma (PPP).
Epinephrine Reagent may be used with human or animal Platelet Rich Plasma (PRP) for routine platelet aggregation tests. Results are based on the concentration, extent, and rate of aggregation compared to a Platelet Poor Plasma (PPP) blank.
In Vitro Diagnostic
Epinephrine Reagent is an in vitro diagnostic reagent intended for Professional Laboratory Use Only. This Reagent is not intended for injection or ingestion.
Test Principle
When introduced to a stirred, 37°C Platelet Rich Plasma (PRP) test sample, exogenous Reagents such as Epinephrine stimulate platelets, prompting them to undergo shape change and aggregate. This initial aggregation is called primary aggregation and is reversible. However, normal platelets possess the ability to release endogenous ADP from their granules, leading to a secondary, irreversible wave of aggregation. The Light Transmission Platelet Aggregometer effectively captures these changes by displaying parameters such as lag phase, shape change, and the rate and extent of aggregation over a predetermined testing period.
Hyper-reactivity may be demonstrated. If so, the Sticky Platelet Procedure should be followed for confirmation. Not all healthy people will respond to the Epinephrine Reagent.
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