EPINEPHRINE REAGENT Adrenaline

Epinephrine Reagent's Pivotal Role in Platelet Studies for Hypersensitivity and Sticky Platelet Syndrome Assessment

Epinephrine Reagent (Adrenaline) is for routine use in platelet studies for the evaluation of hypersensitivity of platelets in Platelet Rich Plasma and evaluation of platelet responses to a weak agonist.

Induces Platelet Aggregation and Serotonin Release
Produces Secondary Aggregation Wave when Alpha Granules Release ADP
Potentiates Aggregation by Other Agonists
Used in Testing for Sticky Platelet Syndrome
Does Not Induce Shape Change

Catalog No.: 101311

Net Contents: 3 x 0.5 mL

Epinephrine Reagent, also known as Adrenaline, serves as a routine component in platelet studies, offering a valuable tool for assessing the hypersensitivity of platelets within Platelet Rich Plasma (PRP) and evaluating their responses to weak agonists. When introduced to platelet-rich plasma, Epinephrine plays a pivotal role in stimulating platelets, inducing what is commonly termed as primary aggregation.

This process triggers normal platelets to undergo further responses, leading to the release of endogenous ADP from alpha granules. The consequential release of endogenous ADP gives rise to a secondary aggregation wave, providing researchers with a comprehensive view of platelet behavior. The reagent's ability to produce this secondary aggregation wave is particularly noteworthy, shedding light on the intricate dynamics of platelet function.

Epinephrine Reagent has been identified as a potent facilitator of platelet aggregation by other agonists, thereby amplifying the overall understanding of platelet responses. This attribute enhances the versatility of Epinephrine Reagent in the field of platelet studies, enabling researchers to explore a wide range of scenarios and stimuli.

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Discover Expected Results Chart

Moreover, the reagent finds practical application in the testing for Sticky Platelet Syndrome, offering a diagnostic tool for this particular platelet disorder. In the context of Sticky Platelet Syndrome testing, Epinephrine Reagent proves instrumental in unveiling distinctive platelet characteristics associated with this syndrome.

Importantly, it's worth noting that Epinephrine Reagent does not induce shape change in platelets during the evaluation process. This unique characteristic adds another layer of precision to its application in platelet studies, allowing researchers to focus specifically on aggregation dynamics without confounding variables related to shape alterations.

Epinephrine Reagent emerges as a versatile and indispensable tool in platelet studies, contributing significantly to the understanding of hypersensitivity, platelet responses, and the intricate mechanisms involved in disorders such as Sticky Platelet Syndrome.

Please explore the provided documentation on this page, including the Epinephrine Reagent Product Data Sheet and Routine Agonist Expected Results Chart. Additionally, consult the Epinephrine Reagent Instruction for Use (IFU) and the Epinephrine Reagent Safety Data Sheet (SDS), both available in six languages. For our European and United Kingdom customers and distributors, thoroughly review the EU and UK Declaration of Conformity to ensure compliance and a nuanced understanding tailored for these specific regions. Acquiring this information is vital for obtaining a comprehensive understanding of the product details and specifications.

Bio/Data Corporation’s Aggregation Reagents: Accessible Safety Data Sheets for Secure Handling

Bio/Data Corporation provides comprehensive Safety Data Sheets (SDS) for each of its carefully formulated aggregation reagents. These essential documents ensure safe handling, storage, and spill management while offering clear instructions on potential hazards. Designed for easy viewing, saving, and printing, the SDS are readily accessible to support a safe work environment and responsible chemical management.

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Product Description

Epinephrine Reagent is a stabilized and lyophilized preparation of L-Adrenaline that activates the GP IIa adreno receptor causing platelet aggregation without shape change. Although it can enhance the response of platelets to other agonists, Epinephrine Reagent is a weak (reversible) agonist. It may or may not elicit a response in healthy people. Epinephrine Reagent has been optimized for use with Light Transmission Aggregometers. It may also be used with other turbidometric or impedance analyzers, and flow cytometers.

Review Instructions For Use

Intended Purpose

Epinephrine Reagent (Adrenaline) is for routine use in platelet studies for the evaluation of hypersensitivity of platelets in Platelet Rich Plasma (PRP) and platelet responses to a weak agonist

Detection / Measurement

Epinephrine Reagent is used, in conjunction with other diluents and control samples, to measure changes of the light transmission in a Platelet Rich Plasma (PRP) test sample.

Product Function

Epinephrine Reagent provides insight into different aspects of platelet function / quality. This Reagent aids in accessing various acquired and inherited platelet disorders or the efficacy of anti-platelet therapies.

Test Principle

When introduced to a stirred, 37°C Platelet Rich Plasma (PRP) test sample, exogenous Reagents such as Epinephrine stimulate platelets, prompting them to undergo shape change and aggregate. This initial aggregation is called primary aggregation and is reversible. However, normal platelets possess the ability to release endogenous ADP from their granules, leading to a secondary, irreversible wave of aggregation. The Light Transmission Platelet Aggregometer effectively captures these changes by displaying parameters such as lag phase, shape change, and the rate and extent of aggregation over a predetermined testing period. Hyper-reactivity may be demonstrated. If so, the Sticky Platelet Procedure should be followed for confirmation. Not all healthy people will respond to the Epinephrine Reagent.

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Reagents Provided

Epinephrine Reagent, 3 x 0.5mL.

Reagents and Materials Required but Not Provided

Purified Water (Distilled, Deionized, Reagent Grade), pH 5.3 – 7.2 for reconstitution
TRIS Buffered Saline (TBS) or 0.85% physiological saline for dilutions

NOTE: USING BLOOD BANK SALINE WILL CAUSE ERRONEOUS RESULTS.

Materials and Accessories

Platelet Aggregometer
Centrifuge
Electronic Pipette
Pipette Tips
Aggregometer Test Tubes (Siliconized)
Aggregometer Stir Bars (Plastic Coated)
Plastic Sample Tubes and Caps (For Dilutions)

Reagent Storage

Epinephrine Reagent does not require temperature protection during shipment.
Upon receipt, store Epinephrine Reagent at 2 – 8° C in its original packaging.
Reconstituted Epinephrine Reagent is stable for 30 days when stored in its’ tightly capped, original container at 2 – 8° C.

Specific Information Provided

Epinephrine Reagent is not intended for the detection of a specific disorder, condition, or risk factor.

Epinephrine Reagent plays a pivotal role in platelet activation and aggregation. Upon binding to specific receptors on the platelet surface, particularly α2-adrenergic receptors, epinephrine initiates intracellular signaling cascades. This cascade induces rapid changes in platelet shape and triggers the release of calcium ions, crucially mediated through α2-adrenergic receptor activation. The sustained response, essential for stable aggregation, is facilitated by α2-adrenergic receptor activation. Epinephrine Reagent is instrumental in precisely stimulating platelet activation and aggregation by interacting with these adrenergic receptors. Observing platelet aggregation in response to Epinephrine Reagent allows clinicians to assess and evaluate platelet function / quality and disorders associated with abnormalities in platelet activation and aggregation. This process is pivotal for comprehending clot formation dynamics and evaluating the effectiveness of anti-platelet therapies in preventing thrombotic events. Epinephrine prompts the release of secondary mediators, further amplifying platelet activation and aggregation.

Bio/Data Corporation's Commitment to IVDR Compliance
Bio/Data Corporation proudly complies with the stringent EU In Vitro Diagnostic Regulation (IVDR) 2017/746 and other relevant legislation. Through its Declaration of Conformity (DOC), the company ensures that its devices meet essential health, safety, and environmental standards.

Bio/Data Corporation's Commitment to UKCA Compliance
Bio/Data Corporation ensures product excellence by strictly adhering to UKCA regulations. The company's Declaration of Conformity (DOC) affirms its dedication to meeting health, safety, and environmental standards outlined in UKCA directives and any related updates, demonstrating its unwavering commitment to compliance.

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