COLLAGEN REAGENT Soluble Calf Skin, Type 1

Collagen Reagent (Soluble Calf Skin, Type 1) Unleashes Innovative Insights into Platelet Rich Plasma Responses

Collagen Reagent (Soluble Calf Skin, Type 1) is intended for routine use in inducing Platelet Rich Plasma responses to activation, aggregation and inhibition.

Displays LAG PHASE Prior to Platelet Shape Change
Acts as a Substrate for Platelet Adhesion
Induces Platelet Activation and Aggregation
Causes Integrin Activation via GP VI Receptor
Releases Thrombotic Substances
Generates TX A2

Catalog No.: 101562

Net Contents: 3 x 0.5 mL

Collagen Reagent (Soluble Calf Skin, Type 1) serves as a pivotal tool in the realm of platelet research, offering a reliable methodology for inducing and studying Platelet Rich Plasma (PRP) responses encompassing activation, aggregation, and inhibition.

In the initial stages, upon the introduction of collagen to platelet-rich plasma, a lag phase is observed before a significant alteration in platelet shape occurs. This lag phase provides a crucial temporal window for researchers to closely observe and analyze the dynamic changes in platelet behavior.

Functioning as a substrate, the collagen reagent actively facilitates platelet adhesion, creating an essential foundation for subsequent biological processes. As platelets adhere to the collagen, a cascade of events is triggered, leading to their activation and subsequent aggregation. This orchestrated series of responses not only sheds light on fundamental platelet biology but also serves as a valuable tool for investigating potential therapeutic interventions related to platelet function.

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Moreover, the collagen reagent plays a pivotal role in integrin activation through the GP VI receptor, further elucidating the molecular mechanisms involved in platelet responses. This in-depth understanding of integrin activation contributes to the broader comprehension of platelet physiology and its implications in various physiological and pathological conditions.

An additional facet of the collagen reagent's impact lies in its ability to release thrombotic substances, adding a layer of complexity to the study of platelet function. This capability broadens the scope of research, enabling scientists to explore the intricate interplay of molecular signals and substances involved in thrombosis.

Furthermore, the collagen reagent stands as a generator of TX A2, a biologically active substance with implications for platelet function. This specific generation adds a nuanced dimension to the study of platelet responses, allowing researchers to delve deeper into the signaling pathways and molecular players involved in the intricate balance of hemostasis and thrombosis. In essence, the routine use of Collagen Reagent (Soluble Calf Skin, Type 1) not only induces a spectrum of platelet responses but also opens up new avenues for advancing our understanding of platelet biology and its relevance to health and disease.

Kindly examine the documentation available on this page, which includes the Collagen Reagent Product Data Sheet and Routine Agonist Expected Results Chart. Furthermore, refer to the Collagen Reagent Instruction for Use (IFU) and the Collagen Reagent Safety Data Sheet, both accessible in six languages. For our customers and distributors in Europe and the United Kingdom, carefully review the Collagen Reagent EU Declaration of Conformity and the Collagen Reagent UK Declaration of Conformity to ensure compliance and a detailed understanding tailored to these specific regions. This information is crucial for achieving a comprehensive grasp of the product details and specifications.

Bio/Data Corporation’s Aggregation Reagents: Accessible Safety Data Sheets for Secure Handling

Bio/Data Corporation provides comprehensive Safety Data Sheets (SDS) for each of its carefully formulated aggregation reagents. These essential documents ensure safe handling, storage, and spill management while offering clear instructions on potential hazards. Designed for easy viewing, saving, and printing, the SDS are readily accessible to support a safe work environment and responsible chemical management.

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Product Description

Collagen Reagent is a lyophilized preparation of Soluble Calf Skin (Type 1). Collagen Reagent induces platelet shape change and activates platelets. The activated platelets then release thrombotic compounds from their granules, which serve to recruit additional platelets to an injury site. Collagen Reagent has been optimized for use with Light Transmission Aggregometers. It may also be used with other turbidometric or impedance analyzers, and flow cytometers.

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Intended Purpose

Collagen Reagent (Soluble Calf Skin, Type 1) is intended for routine use in inducing Platelet Rich Plasma (PRP) responses to activation, aggregation, and inhibition.

Detection / Measurement

Collagen Reagent is used, in conjunction with other diluents and control samples, to measure changes of the light transmission in a Platelet Rich Plasma (PRP) test sample.

Product Function

Collagen Reagent provides insight into different aspects of platelet function / quality. This Reagent aids in accessing various acquired and inherited platelet disorders or the efficacy of anti-platelet therapies.

Test Principle

When introduced to a stirred, 37°C Platelet Rich Plasma (PRP) test sample, exogenous Reagents such as ADP, Arachidonic Acid, Collagen, Epinephrine, and Ristocetin stimulate platelets, prompting them to undergo shape change and aggregate. This initial aggregation is called primary aggregation and is reversible. However, normal platelets possess the ability to release endogenous ADP from their granules, leading to a secondary, irreversible wave of aggregation. The Light Transmission Platelet Aggregometer effectively captures these changes by displaying parameters such as lag phase, shape change, and the rate and extent of aggregation over a predetermined testing period.

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Reagents Provided

Collagen Reagent, 3 x 0.5mL.

Reagents and Materials Required but Not Provided

Purified Water (Distilled, Deionized, Reagent Grade), pH 5.3 – 7.2 for reconstitution
TRIS Buffered Saline (TBS) or 0.85% physiological saline for dilutions

NOTE: USING BLOOD BANK SALINE WILL CAUSE ERRONEOUS RESULTS.

Materials and Accessories

Platelet Aggregometer
Centrifuge
Electronic Pipette
Pipette Tips
Aggregometer Test Tubes (Siliconized)
Aggregometer Stir Bars (Plastic Coated)
Plastic Sample Tubes and Caps (For Dilutions)

Reagent Storage

Collagen Reagent does not require temperature protection during shipment.
Upon receipt, store Collagen Reagent at 2 – 8° C in its original packaging.
Reconstituted Collagen Reagent is stable for 30 days when stored in its’ tightly capped, original container at 2 – 8° C.

Collagen Normal Platelet Aggregation Results

Specific Information Provided

Collagen Reagent is not intended for the detection of a specific disorder, condition, or risk factor.

Collagen Reagent initiates platelet activation and aggregation. Upon binding glycoprotein receptors on the platelet surface, particularly glycoprotein VI (GP VI), Collagen sets off intracellular signaling cascades. This triggers rapid changes in platelet shape and the release of calcium ions through GP VI receptors, with sustained activation facilitated by integrin α2β1, ensuring stable aggregation. Utilized to precisely stimulate platelet activation and aggregation, Collagen Reagent interacts with these receptors, providing a means for clinicians to assess platelet function / quality and disorders linked to collagen-induced platelet activation abnormalities. This process is vital for comprehending clot formation dynamics and evaluating the efficacy of anti-platelet therapies inhibiting thrombotic events. Collagen prompts the release of secondary mediators, further amplifying platelet activation and aggregation.

Bio/Data Corporation's Commitment to IVDR Compliance
Bio/Data Corporation proudly complies with the stringent EU In Vitro Diagnostic Regulation (IVDR) 2017/746 and other relevant legislation. Through its Declaration of Conformity (DOC), the company ensures that its devices meet essential health, safety, and environmental standards.

Bio/Data Corporation's Commitment to UKCA Compliance
Bio/Data Corporation ensures product excellence by strictly adhering to UKCA regulations. The company's Declaration of Conformity (DOC) affirms its dedication to meeting health, safety, and environmental standards outlined in UKCA directives and any related updates, demonstrating its unwavering commitment to compliance.

View EU Declaration of Conformity