COLLAGEN REAGENT Soluble Calf Skin, Type 1

Collagen Reagent (Soluble Calf Skin, Type 1) Unleashes Innovative Insights into Platelet Rich Plasma Responses

Collagen Reagent (Soluble Calf Skin, Type 1) is intended for routine use in inducing Platelet Rich Plasma responses to activation, aggregation and inhibition.

Displays LAG PHASE Prior to Platelet Shape Change
Acts as a Substrate for Platelet Adhesion
Induces Platelet Activation and Aggregation
Causes Integrin Activation via GP VI Receptor
Releases Thrombotic Substances
Generates TX A2

Catalog No.: 101562

Net Contents: 3 x 0.5 mL

Collagen Reagent is a lyophilized preparation of Soluble Calf Skin (Type 1). Collagen Reagent induces platelet shape change and activates platelets. The activated platelets then release thrombotic compounds from their granules, which serve to recruit additional platelets to an injury site.

Collagen Reagent has been optimized for use with Light Transmission Aggregometers. It may also be used with other turbidometric or impedance analyzers, and flow cytometers.

Explore Product Data Sheet

Kindly examine the documentation available on this page, which includes the Collagen Reagent Product Data Sheet, the Collagen Reagent Instruction for Use (IFU), and the Collagen Reagent Safety Data Sheet, accessible in seven languages. For our customers and distributors in Europe and the United Kingdom, carefully review the Collagen Reagent EU Declaration of Conformity and the Collagen Reagent UK Declaration of Conformity to ensure compliance and a detailed understanding tailored to these specific regions. This information is crucial for achieving a comprehensive grasp of the product details and specifications.

Bio/Data Corporation’s Aggregation Reagents: Accessible Safety Data Sheets for Secure Handling

Bio/Data Corporation provides comprehensive Safety Data Sheets (SDS) for each of its carefully formulated aggregation reagents. These essential documents ensure safe handling, storage, and spill management while offering clear instructions on potential hazards. Designed for easy viewing, saving, and printing, the SDS are readily accessible to support a safe work environment and responsible chemical management.

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Product Function

Collagen Reagent provides insight into different aspects of platelet function / quality. This Reagent aids in assessing various acquired and inherited platelet disorders or the efficacy of anti-platelet therapies.

Review Instructions For Use

Specific Information Provided
Collagen Reagent is not intended for the detection of a specific disorder, condition, or risk factor.

Collagen Reagent initiates platelet activation and aggregation. Upon binding glycoprotein receptors on the platelet surface, particularly glycoprotein VI (GP VI), Collagen sets off intracellular signaling cascades. This triggers rapid changes in platelet shape and the release of calcium ions through GP VI receptors, with sustained activation facilitated by integrin α2β1, ensuring stable aggregation. Utilized to precisely stimulate platelet activation and aggregation, Collagen Reagent interacts with these receptors, providing a means for clinicians to assess platelet function / quality and disorders linked to collagen-induced platelet activation abnormalities. This process is vital for comprehending clot formation dynamics and evaluating the efficacy of anti-platelet therapies inhibiting thrombotic events. Collagen prompts the release of secondary mediators, further amplifying platelet activation and aggregation.

Automation
Collagen Reagent is intended for use in semi-automated and automated Light Transmission Platelet Aggregometers. This Reagent may also be used with other turbidometric or impedance analyzers, and flow cytometers.

Quality / Quantity
There are no primary standards for Collagen Reagent. The responses to this reagent are concentration dependent. A known normal donor should be tested with each new lot of Collagen Reagent. Standards organizations classify Collagen-induced platelet aggregation as semi-quantitative or semi-qualitative.

Collagen Reagent comes packaged as 3 x 0.5 mL vials. The working concentration of Collagen is 1.9 mg / mL.

Specimen Type
The test specimen is prepared from sodium citrate anti-coagulated whole blood. The test sample is Platelet Rich Plasma (PRP). The test blank is Platelet Poor Plasma (PPP).

Collagen Reagent may be used with human or animal Platelet Rich Plasma (PRP) for routine platelet aggregation tests. Results are based on the concentration, extent, and rate of aggregation compared to a Platelet Poor Plasma (PPP) blank.

In Vitro Diagnostic
Collagen Reagent is an in vitro diagnostic reagent intended for Professional Laboratory Use Only. This Reagent is not intended for injection or ingestion.

Test Principle
When introduced to a stirred, 37°C Platelet Rich Plasma (PRP) test sample, exogenous reagents such as ADP, Arachidonic Acid, Collagen, Epinephrine, and Ristocetin stimulate platelets, prompting them to undergo shape change and aggregate. This initial aggregation is called primary aggregation and is reversible. However, normal platelets possess the ability to release endogenous ADP from their granules, leading to a secondary, irreversible wave of aggregation. The Light Transmission Platelet Aggregometer effectively captures these changes by displaying parameters such as lag phase, shape change, and the rate and extent of aggregation over a predetermined testing period.

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Reagents Provided

Collagen Reagent, 3 x 0.5mL.

Reagents and Materials Required but Not Provided

Purified Water (Distilled, Deionized, Reagent Grade), pH 5.3 – 7.2 for reconstitution
TRIS Buffered Saline (TBS) or 0.85% physiological saline for dilutions

NOTE: USING BLOOD BANK SALINE WILL CAUSE ERRONEOUS RESULTS.

Materials and Accessories

Platelet Aggregometer
Centrifuge
Electronic Pipette
Pipette Tips
Aggregometer Test Tubes (Siliconized)
Aggregometer Stir Bars (Plastic Coated)
Plastic Sample Tubes and Caps (For Dilutions)

Reagent Storage

Collagen Reagent does not require temperature protection during shipment.
Upon receipt, store Collagen Reagent at 2 – 8° C in its original packaging.
Reconstituted Collagen Reagent is stable for 30 days when stored in its tightly capped, original containers at 2 – 8° C.

Collagen Normal Platelet Aggregation Results

Bio/Data Corporation's Commitment to IVDR Compliance
Bio/Data Corporation proudly complies with the stringent EU In Vitro Diagnostic Regulation (IVDR) 2017/746 and other relevant legislation. Through its Declaration of Conformity (DOC), the company ensures that its devices meet essential health, safety, and environmental standards.

Bio/Data Corporation's Commitment to UKCA Compliance
Bio/Data Corporation ensures product excellence by strictly adhering to UKCA regulations. The company's Declaration of Conformity (DOC) affirms its dedication to meeting health, safety, and environmental standards outlined in UKCA directives and any related updates, demonstrating its unwavering commitment to compliance.

View EU Declaration of Conformity

View UK Declaration of Conformity

COLLAGEN REAGENT Soluble Calf Skin, Type 1

Collagen Reagent (Soluble Calf Skin, Type 1) Unleashes Innovative Insights into Platelet Rich Plasma Responses

Collagen Reagent (Soluble Calf Skin, Type 1) is intended for routine use in inducing Platelet Rich Plasma responses to activation, aggregation and inhibition.

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Displays LAG PHASE Prior to Platelet Shape Change

Acts as a Substrate for Platelet Adhesion

Induces Platelet Activation and Aggregation

Causes Integrin Activation via GP VI Receptor

Releases Thrombotic Substances

Generates TX A2