ADP REAGENT Adenosine-5'-Diphosphate

ADP Reagent Reveals Key Aggregation Platelet Responses

ADP Reagent (Adenosine-5'-Diphosphate) is for routine use in eliciting a concentration dependent activation or aggregation response in a Platelet Rich Plasma sample.

Induces Platelet Shape Change
Causes Primary Platelet Aggregation
Generates Thromboxane A2
Enhances Secondary Aggregation Induced by Granule Release
Indicates Occurrence of Disaggregation Response
Shows Blockade Effects on P2Y1, P2Y12, and P2X1 Receptors
Displays Effects of LVAD on Platelet Function

Catalog No.: 101312

Net Contents: 3 x 0.5 mL

When introduced into platelet-rich plasma, the ADP (Adenosine-5'-Diphosphate) reagent emerges as a powerful catalyst, orchestrating a multifaceted sequence of events within the realm of platelet dynamics. The initial phase of this orchestrated response is marked by a striking transformation in the shape of platelets, coupled with the formation of aggregates.

This primary aggregation, induced by the exogenous administration of ADP, is aptly termed "primary aggregation." Notably, this phase is characterized by its reversible nature, underlining the dynamic adaptability of platelets in the presence of ADP. However, the narrative doesn't end here; normal platelets, in a subsequent maneuver, escalate the complexity of their response by releasing endogenous ADP from their granules.

This release of endogenous ADP serves as a trigger for a secondary wave of aggregation, distinctive in its irreversibility. This secondary aggregation unveils a deeper layer of platelet behavior, shedding light on the nuanced interplay between exogenous and endogenous ADP. The reversible primary aggregation and the irreversible secondary aggregation collectively contribute to a comprehensive understanding of the intricate and sequential facets of platelet behavior in response to ADP stimulation.

Explore Product Data Sheet

Discover Expected Results Chart

This intricate dance of platelets, choreographed by the influence of ADP, not only unravels the immediate responses in platelet shape and aggregation but also underscores the nuanced regulatory mechanisms at play within the dynamic milieu of platelet-rich plasma. As we delve into the intricacies of these responses, we gain a richer perspective on the complexity of platelet biology and the subtle orchestration of events initiated by the introduction of ADP.

Please explore the literature provided, encompassing the ADP Reagent Product Data Sheet, Routine Agonist Expected Results Chart, and the ADP Instruction for Use (IFU), along with the ADP Safety Data Sheet (SDS) — both available in six languages. Additionally, for our European and United Kingdom customers and distributors, review the ADP EU Declaration of Conformity and the ADP UK Declaration of Conformity, to ensure compliance and understanding tailored specifically for these regions. This information is crucial for a comprehensive grasp of the product details and specifications.

Bio/Data Corporation’s Aggregation Reagents: Accessible Safety Data Sheets for Secure Handling

Bio/Data Corporation provides comprehensive Safety Data Sheets (SDS) for each of its carefully formulated aggregation reagents. These essential documents ensure safe handling, storage, and spill management while offering clear instructions on potential hazards. Designed for easy viewing, saving, and printing, the SDS are readily accessible to support a safe work environment and responsible chemical management.

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Product Description
ADP Reagent is a lyophilized preparation of Adenosine-5’-Diphosphate. It is an essential component in platelet aggregation. ADP acts as an agonist or activator, binding to platelet receptors and triggering a series of biochemical events that lead to platelet activation and aggregation. ADP Reagent has been optimized for use with Light Transmission Aggregometers. It may also be used with other turbidometric or impedance analyzers, and flow cytometers.

Review Instructions For Use

Intended Purpose
ADP Reagent (Adenosine-5’-Diphosphate) is for routine use in eliciting a concentration dependent activation or aggregation response in a Platelet Rich Plasma (PRP) test sample.

Detection / Measurement
ADP Reagent is used, in conjunction with other diluents and control samples, to measure changes of the light transmission in a Platelet Rich Plasma (PRP) test sample.

Product Function
ADP Reagent provides insight into different aspects of platelet function / quality. This Reagent aids in accessing various acquired and inherited platelet disorders or the efficacy of anti-platelet therapies.

Test Principle

When introduced to a stirred, 37°C Platelet Rich Plasma (PRP) test sample, exogenous Reagents such as ADP stimulate platelets, prompting them to undergo shape change and aggregate. This initial aggregation is called primary aggregation and is reversible. However, normal platelets possess the ability to release endogenous ADP from their granules, leading to a secondary, irreversible wave of aggregation. The Light Transmission Platelet Aggregometer effectively captures these changes by displaying parameters such as lag phase, shape change, and the rate and extent of aggregation over a predetermined testing period.

READ MORE... Easily Download the ADP Reagent Instructions for Use in Your Selected Language.

Reagents Provided

ADP Reagent, 3 x 0.5mL.

Reagents and Materials Required but Not Provided

Purified Water (Distilled, Deionized, Reagent Grade), pH 5.3 – 7.2 for reconstitution
TRIS Buffered Saline (TBS) or 0.85% physiological saline for dilutions

NOTE: USING BLOOD BANK SALINE WILL CAUSE ERRONEOUS RESULTS.

Materials and Accessories

Platelet Aggregometer
Centrifuge
Electronic Pipette
Pipette Tips
Aggregometer Test Tubes (Siliconized)
Aggregometer Stir Bars (Plastic Coated)
Plastic Sample Tubes and Caps (For Dilutions)

Reagent Storage

ADP Reagent does not require temperature protection during shipment.
Upon receipt, store ADP Reagent at 2 – 8° C in its original packaging.
Reconstituted ADP Reagent is stable for 30 days when stored in its’ tightly capped, original container at 2 – 8° C.

Specific Information Provided

ADP Reagent is not intended for the detection of a specific disorder, condition, or risk factor. ADP Reagent plays a pivotal role in platelet activation and aggregation. When ADP binds to specific receptors on the platelet surface, such as P2Y1 and P2Y12, it initiates intracellular signaling cascades. This activation induces rapid changes in platelet shape and the release of calcium ions through P2Y1 receptors, while P2Y12 activation sustains the response, ensuring stable aggregation. ADP Reagent is utilized to stimulate platelet activation and aggregation precisely by interacting with these ADP receptors. By observing platelet aggregation in response to ADP, clinicians can assess platelet function / quality related to abnormalities in platelet activation and aggregation. This process is crucial for understanding clot formation dynamics and evaluating the efficacy of anti-platelet therapies in preventing thrombotic events. ADP prompts the release of secondary mediators like Thromboxane A2 (TX A2 ), further amplifying platelet activation and aggregation

Bio/Data Corporation's Commitment to IVDR Compliance
Bio/Data Corporation proudly complies with the stringent EU In Vitro Diagnostic Regulation (IVDR) 2017/746 and other relevant legislation. Through its Declaration of Conformity (DOC), the company ensures that its devices meet essential health, safety, and environmental standards.

Bio/Data Corporation's Commitment to UKCA Compliance
Bio/Data Corporation ensures product excellence by strictly adhering to UKCA regulations. The company's Declaration of Conformity (DOC) affirms its dedication to meeting health, safety, and environmental standards outlined in UKCA directives and any related updates, demonstrating its unwavering commitment to compliance.

View EU Declaration of Conformity