Catalog No.: 101297

Net Contents: 3 x 0.5 mL

ARACHIDONIC ACID REAGENT Sodium Arachidonate

Arachidonic Acid Reagent Sheds Light on Thromboxane A2 Activation in Routine Plasma Studies

Arachidonic Acid (Sodium Arachidonate) Reagent is for routine use in demonstrating thromboxane A2 activation response in Platelet Rich Plasma samples.

Stimulates Platelets to Convert COX 1 Enzyme into TX A2
Demonstrates the Efficacy of TX A2 Platelet Activation Pathway
Shows the Increase in Microparticles and Activated Platelets in Response to Increasing Concentrations
May Cause Lysis of Red Blood Cells and Subsequent ADP Dependent Platelet Activation Responses in Platelet Rich Plasma Samples

Arachidonic Acid Reagent is a lyophilized preparation of the Sodium Salt of Arachidonic Acid. It is an essential fatty acid present in the granules of platelets and on the platelet membrane. It is processed in multiple steps and converted to Thromboxane A2 (TX A2). Arachidonic Acid Reagent induces platelet activation and aggregation.

Arachidonic Acid Reagent has been optimized for use with Light Transmission Aggregometers. It may also be used with other turbidometric or impedance analyzers, and flow cytometers.

Explore Product Data Sheet

Please examine the documentation provided on this page, including the Arachidonic Acid Reagent Product Data Sheet, the Arachidonic Acid Instruction for Use (IFU), and the Arachidonic Acid Safety Data Sheet (SDS), which are available in seven languages. For our European and United Kingdom customers and distributors, thoroughly review the Arachidonic Acid EU Declaration of Conformity and the Arachidonic Acid UK Declaration of Conformity to ensure compliance and a nuanced understanding tailored for these specific regions. This information is essential for obtaining a comprehensive grasp of the product details and specifications.

Bio/Data Corporation’s Aggregation Reagents: Accessible Safety Data Sheets for Secure Handling

Bio/Data Corporation provides comprehensive Safety Data Sheets (SDS) for each of its carefully formulated aggregation reagents. These essential documents ensure safe handling, storage, and spill management while offering clear instructions on potential hazards. Designed for easy viewing, saving, and printing, the SDS are readily accessible to support a safe work environment and responsible chemical management.

Access Safety Data Sheet

Product Function

Arachidonic Acid Reagent provides insight into different aspects of platelet function / quality. This Reagent aids in assessing various acquired and inherited platelet disorders or the efficacy of anti-platelet therapies.

Review Instructions For Use

Specific Information Provided
Arachidonic Acid Reagent is not intended for the detection of a specific disorder, condition, or risk factor.

Arachidonic Acid Reagent initiates platelet activation and aggregation through the arachidonic acid pathway. Upon binding to platelet surface receptors, arachidonic acid undergoes enzymatic conversion to Thromboxane A2 (TX A2), facilitating intracellular signaling cascades. This prompts rapid changes in platelet shape and calcium ion release, crucial for stable aggregation. Observing platelet aggregation in response to Arachidonic Acid Reagent allows clinicians to assess and evaluate platelet function / quality, abnormalities, and anti-platelet therapies. Arachidonic Acid Reagent’s induction of secondary mediators like Thromboxane A2 (TX A2) amplifies platelet activation.

Automation
Arachidonic Acid Reagent is intended for use in semi-automated and automated Light Transmission Platelet Aggregometers. This Reagent may also be used with other turbidometric or impedance analyzers, and flow cytometers.

Quality / Quantity
There are no primary standards for the Arachidonic Acid Reagent. The responses to this reagent are concentration dependent. A known normal donor should be tested with each new lot of Arachidonic Acid Reagent. Standards organizations classify Arachidonic Acid induced platelet aggregation as semi-quantitative or semi-qualitative.

Arachidonic Acid Reagent comes packaged as 3 x 0.5 mL vials. The working concentration of Arachidonic Acid is 5 mg / mL.

Specimen Type
The test specimen is prepared from sodium citrate anti-coagulated whole blood. The test sample is Platelet Rich Plasma (PRP). The test blank is Platelet Poor Plasma (PPP).

Arachidonic Acid Reagent may be used with human or animal Platelet Rich Plasma (PRP) for routine platelet aggregation tests. Results are based on the concentration, extent, and rate of aggregation compared to a Platelet Poor Plasma (PPP) blank.

In Vitro Diagnostic
Arachidonic Acid Reagent is an in vitro diagnostic reagent intended for Professional Laboratory Use Only. This Reagent is not intended for injection or ingestion.

Test Principle
When introduced to a stirred, 37°C Platelet Rich Plasma (PRP) test sample, exogenous Reagents such as ADP, Arachidonic Acid, Collagen, Epinephrine, and Ristocetin stimulate platelets, prompting them to undergo shape change and aggregate. This initial aggregation is called primary aggregation and is reversible. However, normal platelets possess the ability to release endogenous ADP from their granules, leading to a secondary, irreversible wave of aggregation. The Light Transmission Platelet Aggregometer effectively captures these changes by displaying parameters such as lag phase, shape change, and the rate and extent of aggregation over a predetermined testing period.

READ MORE... Easily Download the Arachidonic Acid Reagent Instructions for Use in Your Selected Language.

Reagents Provided

Arachidonic Acid Reagent, 3 x 0.5mL.

Reagents and Materials Required but Not Provided

Purified Water (Distilled, Deionized, Reagent Grade), pH 5.3 – 7.2 for reconstitution
TRIS Buffered Saline (TBS) or 0.85% physiological saline for dilutions

NOTE: USING BLOOD BANK SALINE WILL CAUSE ERRONEOUS RESULTS.

Materials and Accessories

Platelet Aggregometer
Centrifuge
Electronic Pipette
Pipette Tips
Aggregometer Test Tubes (Siliconized)
Aggregometer Stir Bars (Plastic Coated)
Plastic Sample Tubes and Caps (For Dilutions)

Reagent Storage

Arachidonic Acid Reagent does not require temperature protection during shipment.
Upon receipt, store Arachidonic Acid Reagent at 2 – 8° C in its original packaging.
Reconstituted Arachidonic Acid Reagent is stable for 24 hours when stored in its tightly capped, original containers at 2 – 8° C.

Arachidonic Acid Normal Platelet Aggregation Results

Bio/Data Corporation's Commitment to IVDR Compliance
Bio/Data Corporation proudly complies with the stringent EU In Vitro Diagnostic Regulation (IVDR) 2017/746 and other relevant legislation. Through its Declaration of Conformity (DOC), the company ensures that its devices meet essential health, safety, and environmental standards.

Bio/Data Corporation's Commitment to UKCA Compliance
Bio/Data Corporation ensures product excellence by strictly adhering to UKCA regulations. The company's Declaration of Conformity (DOC) affirms its dedication to meeting health, safety, and environmental standards outlined in UKCA directives and any related updates, demonstrating its unwavering commitment to compliance.

View EU Declaration of Conformity

View UK Declaration of Conformity

ARACHIDONIC ACID REAGENT Sodium Arachidonate

Arachidonic Acid Reagent Sheds Light on Thromboxane A2 Activation in Routine Plasma Studies

Arachidonic Acid (Sodium Arachidonate) Reagent is for routine use in demonstrating thromboxane A2 activation response in Platelet Rich Plasma samples.

​

Stimulates Platelets to Convert COX 1 Enzyme into TX A2

Demonstrates the Efficacy of TX A2 Platelet Activation Pathway

Shows the Increase in Microparticles and Activated Platelets in Response to Increasing Concentrations

May Cause Lysis of Red Blood Cells and Subsequent ADP Dependent Platelet Activation Responses in Platelet Rich Plasma Samples